End capped c18. Are you interested in real-world .

End capped c18. LiChrospher® 100 RP-18 and RP-18 endcapped columns offer very high reliability for reversed-phase HPLC of acidic, neutral and weakly basic compounds. Stationary Phase Category: C18, octadecylsilane (L1) Ligand Type: End-capped C18 Particle: 1. Properties Product Name C18-Reversed phase silica gel, suitable for column chromatography, fully end-capped In summary, new end-capping technologies can significantly improve the analysis of compounds that were once considered challenging. Packaged in convenient centrifuge tubes, these sorbents streamline your laboratory workflow. Mar 18, 2024 · Do You Know What is c18 Column in HPLC ? Check this Article for Details to Know the Definition, Characteristics, Application of C18 Column. 8 µm, 3. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Available in 50 mm Leverage Waters Corporation's advanced column particle technologies, including BEH, CSH, HSS, and Solid-Core, to achieve superior chromatographic performance across a wide range of applications. Are you interested in real-world Shop Agilent Technologies SampliQ Silica C18 Endcapped SPE Cartridges at Thomas Scientific, your trusted partner in Science. Different endcapping procedures are utilized today in modern HPLC column phases. Part#: CEC1800X Chemical Phase = C18 Irregular particles 40-63 micron Organic Loading = 21. Poroshell 120 EC-C18 and C8 Poroshell 120 SB-Aq • Robust end-capped C18/C8 for best peak shape at pH 2-9 Hypersil™ ODS C18 Columns. All these variations result in differences in retention of different compounds, offering different selectivity between these stationary phases. Enviro-Clean® Endcapped C18 PTFE Frits, Glass Column 1000 mg 6 mL Part Number: EEC181M6G $ 355. 00 Enviro-Clean C18 (Octadecyl) – Endcapped 1000mg / 6mL Glass Column Retain and resolve polar analytes using Thermo Scientific™ Hypersil GOLD™ aQ Polar Endcapped C18 columns. Ideal for acidic conditions. They utilize an embedded amide, similar to ZORBAX Bonus-RP columns, and are available in 3 μm and 5 μm particle sizes. Thermo ScientificTM SyncronisTM C18 HPLC columns show excellent column to column reproducibility, as illustrated here by the analysis of zidovudine using five separate columns. SPE Bulk Sorbent, C18, endcapped, 100 g. CHROMABOND® C18EC Endcapped - SPE Cartridges Properties: Base material silica, pore size 60Å, particle size 45µm pH stability 2-8 Octadecyl phases, end-capped, carbon content 14% Very nonpolar, hydrophobic interactions with a wide variety of organic compounds Advantageous for clean-up of samples with large structural variations (polarity differences) Recommended Applications: Nonpolar compounds End -capped C18 and Not – end – capped The most popular Silica-based sorbent Strongly hydrophobic and non-polar sorbent SPE columns available with powder or SPE disks sorbents Sep 3, 2004 · It's a base-deactivated, end-capped column, but it's a C12, I believe, rather than a C18. Exploring the Unique Characteristics of Agilent InfinityLab Poroshell 120 C18 LC Columns: EC-C18, SB-C18, HPH-C18, AQ-C18, and CS-C18 The InfinityLab Poroshell 120 column range from Agilent has expanded to offer thirteen bonded phases for use with reversed phase LC separations. 5um, 3. It creates strong hydrophobic interactions, which allow it to separate a wide variety of organic compounds effectively. Superspher® RP-18 endcapped (4 µm) columns for reversed-phase HPLC are classified as L1 columns, fully compliant with US Pharmacopoeia specifications. Our experts break down everything you need to know here. Thermo Scientific Hypersil GOLD aQ C18 Polar Endcapped HPLC Columns from Thermo Scientific™. Use this column, with its specially designed solid core, for the separation of compounds in highly aqueous mobile phases. YMC-Pack ODS-A is a high-coverage, fully-endcapped C18 phase which exhibits exceptional lot-to-lot reproducibility. The SS end Properties Product Name C18-Reversed phase silica gel, suitable for column chromatography, fully end-capped form gel form A traditional, end-capped C18 phase, which has about 20% carbon load, is a great place to start if you’re not sure where to begin. This sorbent can retain most organic substances and is widely used in fields such as environmental and food safety. Regarded as having the broadest spectrum of retention among bonded silica sorbents. Silica is an amorphous Synergi Hydro-RP HPLC column is a polar end-capped C18 phase that provides extreme retention for hydrophobic compounds and slight polar selectivity: Extreme retention of hydrophobic compounds Feb 21, 2025 · The end cap can range from a methyl end cap to a polar end cap that creates a C18AQ type column that resists phase collapse in highly aqueous mobile phases. 5um and 5um particles? and What is the difference between XTerra and Xbridge columns? 2. This study presents two straightforward strategies for preparing C18 phases with distinct polar-embedded groups: thioether (C18T) and quaternary ammonium (C18Q). . The peak shape of the alkaline substance is better. HyPURITY AQUASTAR C18 A new member of the HyPURITY family introduced at Pittcon 2003, HyPURITY AQUASTAR columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. Non-endcapped C18 phase with bulky diisobutyl side chains, offers stability for low pH down to pH 1, resisting degradation from aggressive mobile phases. Are you interested in real-world An "endcapped column" is a column that contains chromatographic chemistry which undergoes an additional reaction after ligand bonding to "cap" off any free silica silanols that remain unreacted from the ligand bonding step. 0 mm dimensions. Designed for high-performance liquid chromatography (HPLC). Characteristics of Ecosil C8 Reversed-phase columns: Recommended for HPLC separations of higher hydrophobic samples and samples with strong retention on C8 stationary phase. 00 Add to cart The general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic. Ascentis ® Express C18 is the first choice when starting a new separation method. Aqua is a conventional reversed phase HPLC media with polar endcapping that provides increased retention of polar small molecules and 100% aqueous stability. Feb 8, 2025 · New stationary phases including polar embedded stationary phases that are capable of utilising green mobile phases have recently emerged. Description Phenomenex Synergi 10 µm Hydro-RP 80 Å, LC Column 250 x 50 mm This item comes with a 30-day Repair or Replace warranty. The pH range for this packing is 1-12. End-capped C18 columns have the advantage of offering the highest hydrophobic retention among C18 phases, while limiting other chromatographic interactions, due to end-capping and high surface coverage. UHPLC Columns 5982-1365 C18 Endcapped Box, 30 x 6 mL tubes, 500 mg SampliQ Solid Phase Extraction (SPE) Add to Favorites A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. Hypersil™ BDS C18 Columns. nullPhysical Characteristics Chart This chart provides an overview of Restek’s full offering of HPLC and UHPLC columns, specialty columns, and large molecule columns. C18 is the most popular SPE sorbent because of its extreme retention of nonpolar compounds. After Jan 8, 2015 · European Pharmacopoeia (EU) methods tend to be vague in their description for column requirements, which can make choosing the correct column a little tricky. 8, 2. Feb 22, 2002 · Synthesis and characterization of endcapped C18 stationary phases using a silica hydride intermediate Joseph J Pesek , Maria T Matyska , Raymond J Yu Show more Add to Mendeley Clean Screen® RSV Endcapped C18 Column 80mg 3mL $ 183. The polar endcapped C18 stationary phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. SKU Agilent Technologies ZORBAX HPLC Column, StableBond SB-C18, Reversed Phase, USP: L1, End-Capped: No, 3,0 x 150 mm, 5 µm, 80 Å Article no. Alternative selectivity to C18 with excellent peak shape and sensitivity Hypersil GOLD PFP (pentafluorophenyl) columns build on the performance of Hypersil GOLD silica by providing excellent peak shapes while also offering alternative selectivity in reversed-phase chromatography compared to alkyl chain phases. C18 column compatible with low pH and high temperature Excellent stability and peak shape at highly acidic conditions Traditional, fully porous particles available in 1. To simply things, I listed the Restek HPLC columns that best match the columns (phases) listed in the European Pharmacopoeia “Reagents” chapter. 8 µm, 3 μm, or 5 μm fully porous silica Pore Size: 100 Å Carbon Load: 20% End We would like to show you a description here but the site won’t allow us. C18 columns are ideally used for acidic, basic and neutral analytes in reversed phase mode, e. The sorbent is not end-capped, and secondary silanol interactions can be exploited to enhance extract purity Dec 21, 2021 · ANSWER XBridge BEH C18 packing is made with fully porous, 130A, Ethylene Bridged Hybrid (BEH) particles with tri-functionally bonded C18 ligands and is fully endcapped. Columns come in 316L stainless steel (SS) tubing hardware. Compatible with chromatography systems. 2 Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against : Reagents and Standards for Analytical Chemistry Laboratory Use Plastic container. Gemini C18 HPLC phase offers unique reversed phase selectivity, high structural integrity, and increased loadability which make it ideal for preparative and purification applications in pre-packed columns and bulk media. The polar end-capped C18 phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Silica-based reversed-phase sorbent for hydrophobic interactions. 00 Add to cart Retain and resolve polar analytes using Thermo Scientific™ Hypersil GOLD™ aQ Polar Endcapped C18 columns. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. ISOLUTE® C18 is the most commonly used C18 sorbent for the extraction of acidic, neutral and basic compounds from aqueous sample matrices. 6 x 150 mm, 5 µm, 400 bar. These columns provide excellent peak shape, even with challenging reversed phase separations using highly aqueous mobile phases. The Polar C18 chemistry has been proven to best retain compounds such as metabolites, and XB-C18 chemistry has shown excellent The most retentive of the alkyl-bonded phases, C18 columns are broadly used for pharmaceuticals, steroids, fatty acids, phthalates, environmental analyses and more The polar end-capped C18 phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. The Fused-Core ® particle technology of Ascentis ® Express C18 enables high speed and high resolution separations on HPLC and UHPLC instruments. : 883975-302 1. Wide pH range (2–8) provides excellent data quality for many applications, matrices, and compounds. To understand end-capping, we need to step back and look at the bonded phase on the HPLC column. Oct 28, 2025 · Work in a chromatography lab? Make sure you understand what endcapping is in HPLC columns. Robust bonding In summary, new end-capping technologies can significantly improve the analysis of compounds that were once considered challenging. Phase: Polar end-capped C18 Particle Size: 10 µm Pore Size: 80 Å Surface Area: 475 m²/g Carbon Load: 19% Stability: Stable in 100% aqueous mobile A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. Shop Thermo Scientific™ Hypersil Enviro-Clean® Endcapped C18 PTFE Frits 1000 mg 6 mL Part Number: EEC181M6 $ 152. Silica-based reversed-phase sorbent for strong hydrophobic interactions; ideal for general cleanup of non-polar and moderately polar compounds. Strata C18-E can be best used for the extraction of hydrophobic or polar organic analytes from aqueous matrices. 5 µm, and 5 µm sizes Guards extend the lifetime of analytical columns without diminishing performance USP L1 classification ZORBAX StableBond also includes SB-Aq, SB-C8, SB-C3, SB-Phenyl, and SB-CN for alternative In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. For new methods, upgrade to Luna Omega Polar C18 for small molecules or Biozen columns for peptides and peptide mapping. Oct 27, 2022 · Compared with end-capped Welchrom® C18E, Welchrom® C18 is a general-purpose stationary phase for the extraction of polar and non-polar compounds, but C18 has the ability to retain most organic substances in water-soluble matrices, so it is less selective, It is often used to process analytes with multiple structures or very different structures. For a robust, general-purpose column to use in a wide range of applications, choose Hypersil BDS C18 HPLC Columns. Agilent Polaris Amide-C18 columns provide subtle alternative selectivity because they do not have steric protection. 7, and 5 µm SPP core-shell silica. This HPLC column is packed with endcapped C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. [1] Endcapped columns show decreased retention for hydrogen bond acceptors, such as ionized bases, and increased retention for protonated bases. I read about ACT ACE AQ and I guess there's a ton of other similar phases available. The most hydrophobic, bonded silica sorbent in the Bond Elut range. [2] Selectrasorb™ Bulk Sorbent Endcapped C18 100 g Part Number: CEC1800C $ 364. The alkaline C18 silica gel column is a new generation of different selective C18 developed by Hawach. Performance Benefits: Unique, non-end-capped C18 chemistry designed specifically for method development scientists. Hawach C18-aqueous HPLC column has excellent column peak shapes for basic and polar compounds ensured by the fully end-capped stationary phase. Purospher STAR RP-18 endcapped HPLC columns offer outstanding stability from pH 1. Bond Elut C18 Solid Phase Extraction (SPE) cartridge is the most hydrophobic, bonded silica sorbent available in the Bond Elut SPE family. Endcapping refers to the replacement of available silanol groups in a bonded stationary phase with TMS. Selectrasorb™ Bulk Sorbent Endcapped C18 1 Kg Part Number: CEC1800K $ 2,171. The Bond Elut C18 SPE cartridge is considered to have the broadest retention spectrum among bonded silica sorbents. Don't know if that will disqualify it for you, but it's worked well for us. For example, nowadays, great peak shape and shorter run times can be achieved for the analysis of basic drugs using PS-C18 columns. Easily compare column silica specifications, applications, chromatography properties, similar phases, and USP codes. ISOLUTE C18 (EC) is a retentive, non-polar sorbent for extraction of a range of analytes from aqueous samples using a non-polar retention mechanism. Endcapped columns are 100% aqueous compatible and excellent for separating and retaining polar analytes. Classically end capped phases are slightly more hydrophobic and unpolar than the not end capped phases. 6, 5µm) This asks for "organosilica polymer", so this is no ordinary silica column but a hybride column. The separation has high selectivity and efficiency. Reduce silanol activity and protect the silica from dissolution by endcapping the HPLC columns. Strata C18-E is a silica-based end-capped C18 solid phase extraction (SPE) sorbent that offers strong hydrophobic retention with negligible secondary polar interactions from active silanol groups. Dec 8, 2023 · This technical article discusses what is meant when a column is referred to as end-capped, and what the function of the end-capping is. Fully end-capped and high stability make a good reproducibility for each column. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns. 5 under various mobile phase and temperature conditions. UHPLC Columns This HPLC column is packed with endcapped C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. ISOLUTE C18 (EC) is used for the extraction of acidic, neutral and basic compounds from aqueous sample matrices. Endcapped C18 96 well plate with 100mg silica sorbent for cleanup of non-polar and moderately polar compounds in lab applications. One more question: Has anyone tried UPLC column from C18 silica gel is the most widely used reversed phase material because it is both popular and versatile. For a general purpose C18 phase to use in a broad range of applications, choose Hypersil ODS C18 HPLC Columns. Hopefully this makes it a little easier to choose the correct column for your particular Kinetex® Selectivities Balanced C18 phase that provides the highest degree of hydrophobic selectivity relative to the other Kinetex phases Aromatic and moderate hydrophobic selectivity results in the great retention and separation of aromatic hydrocarbons All purpose phase that offers the hy-drophobic retention and methylene selectivity chromatographers expect from a C18 column C18 phase with protective butyl side chains for improved peak shape for basic compounds under neutral and acidic conditions Aug 1, 2025 · A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. Endcapped C18 column, 500mg 6mL, ideal for general cleanup of compounds. Retain and resolve polar analytes using these polar endcapped C18 columns, which are ideal for use with highly aqueous mobile phases. Available in 50 mm Silica gel-based columns with C18 modification are available with or without end capping. The SS end Jun 25, 2007 · Can anyone tell me the difference between polar encapped and polar embededed columns? Are polar embedded columns more resistant to IP agents contamination or which column should have the longest lifetime? I was using a polar encapped column to a HPIPC aplication with 10 mM DBAA and after cca 200 injections, it went irreversibly contaminated. Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns. Offers the highest hydrophobic retention of any Raptor phase. Reversed-phase HPLC columns usually comprise a silica particle with a stationary phase, such as a C18 hydrocarbon bonded onto the surface. Thermo Scientific QuEChERS Silica Sorbent with C18 (endcapped) in Centrifuge Tubes are designed for efficient sample cleanup in QuEChERS extractions. 7% Average Pore Size = 60Å Surface Area = 500 m2/g Pore Volume = 0. Retain and resolve polar analytes using Thermo Scientific™ Hypersil GOLD™ aQ Polar Endcapped C18 columns. 00 Enviro-Clean C18 (Octadecyl) – Endcapped 1000mg / 6mL Spoiled for choice Want a C18 column that's right for your application? Thermo Scientific C18 columns are available with a range of characteristics to provide exceptional performance. 5982-1365 - C18 Endcapped Box 30x6ml Tubes, 500mg SPE: High-performance chromatography column for precision testing. The sorbent is efficiently end-capped to minimise secondary silanol interactions. The pre-measured sorbent ensures consistent performance, while the endcapped C18 provides enhanced retention of non-polar compounds. Sulphonamides Anabolic steroids Anti-psychotics Beta Retain and resolve polar analytes using Thermo Scientific™ Hypersil GOLD™ aQ Polar Endcapped C18 columns. Dec 21, 2021 · For additional information, see Are BEH C18 1. The Polar C18 chemistry has been proven to best retain compounds such as metabolites, and XB-C18 chemistry has shown excellent I was using Phenomenex Luna Omega Polar C18 years ago, this (or similar solid core particle) would be my go to if I dont get better recommendations. Feb 28, 2013 · Difference between the normal and end capped columns Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques. 00 Add to cart 1 2 3 4 … 10 11 12 → Sep 15, 2022 · What is end capping in HPLC column? Endcapping of the HPLC Column bonded phase minimizes silanol interaction and protects the silica support from dissolution. Copure® C18 Endcapped Octadecyl Copure ® C18 Endcapped Octadecyl (C18) is an octadecyl silica gel that retains non-polar compounds through strong hydrophobic interactions. Extremely retentive for nonpolar compounds. The two strategies were assessed based on convenience, cost, yield, and time efficiency. All Hypersil BDS columns offer the same high-quality base deactivated surface chemistry with endcapping to minimize peak tailing, even for basic drugs. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control. 77 cm3/g Hypersil BDS columns are available in four bonded phases: C18, C8, Cyano and Phenyl functionality. Offers unique Selectivity for Bases (SB) when operating under low-pH conditions. The polar endcapped C18 stationary phase HyPURITY AQUASTAR C18 A new member of the HyPURITY family introduced at Pittcon 2003, HyPURITY AQUASTAR columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. 6μm solid-core particles enable fast, high-efficiency separations at low backpressures. The ODS C18 HPLC column produced by Hawach uses ultra-pure silica gel, it's one of the most versatile C18 ODS analytical columns on the market. g. 7um particles made of the same chemistry as BEH C18 2. Apr 5, 2012 · - stationary phase: end-capped polar-embedded octadecylsilyl amorphous organosilica polymer R (150 x 4. Part of Restek’s Raptor LC column line featuring 1. Rugged 2. 5 to 10. Bottle 5982-1382 C18 Endcapped SPE Bulk Sorbent 25 g 5982-5752 C18 Endcapped Bulk Sorbent 100 g G3920-60005 C18-EC 1 kg : None known. It is available in three different pore sizes and is suitable for the separation of a wide range of compounds. A SS female end fitting with a SS and PEEK frit system keeps the stationary phase in place and packed bed from disturbance. Nov 1, 2023 · Column performance is dependent on how well the bonded phase is functionalized to the silica gel - the amount of C18 groups on the phase or carbon load, the bonding type of the C18 group, and how well residual silanols have been end-capped. 6 mm Base deactivation process Understanding C18 column selectivity: The role of silica surface and bonding density The popularity of columns packed with C18 derivatized silica is due to their wide breadth of application, encompassing non-polar and neutral, acidic, and basic analytes. These columns are broadly used in a wide variety of applications including pharmaceuticals, steroids, fatty acids, phthalates, environmental and more. 10 µm particle size and 250 x 50. May 1, 2003 · C18 rarely offers retention advantages, and selectivity may not be optimum with little retention C8 often provides a little more retention than C18 with similar selectivity Phenyl most retentive with changes in selectivity C3 similar to Phenyl in retention but some changes in selectivity from C18, C8 and Phenyl CN least retentive, definite Hypersil BDS C18 column, 5 μm, 150 x 4. ISOLUTE® C18 is a retentive, non-polar sorbent for extraction of a range of analytes from aqueous samples using a non-polar retention mechanism. ZORBAX StableBond C18 Column, 4. Sep 30, 2025 · Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH. ldmi edl pfm 8z drb0rb q3utbb yxl7zjr nyph vwlh hlsx4t7d

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